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General Protocol / Real-Time Assay
- Add 10 μl of CellTox Green DNA binding dye per 5 ml of cell suspension in culture medium prior to seeding cells in the assay plates. ...
- Mix the tube containing the suspension of cells by inversion or gently vortex to ensure dye homogeneity prior to seeding into assay plates.
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May 1, 2013 · This chapter is an introductory overview of the most commonly used assay methods to estimate the number of viable cells in multi-well plates ...
A protocol for measuring changes in membrane integrity that occur as a result of cell death. The assay assesses cytotoxicity in cell culture after ...
The MTT assay is used to measure cellular metabolic activity as an indicator of cell viability, proliferation and cytotoxicity. This colorimetric assay is ...
Detailed instructions on reagent preparation and assay protocol for an MTT assay to measure cell proliferation or cell cytoxicity.
A protocol for quantitative measurement of lactate dehydrogenase (LDH), a stable cytosolic enzyme that is released upon cell lysis. This assay is a ...
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A dual-color assay for determining cell toxicity by flow cytometry. A green fluorescent cellular stain, CFSE, labels target cells and the red live/dead ...
This assay is commonly used to evaluate the cytotoxic effects of drugs, chemicals, or experimental conditions on cell cultures. It provides a quantitative ...
Aug 12, 2016 · Cell Viability is essential for checking effectiveness of cytotoxic compounds. This guide describes the MTT Assay, Trypan Blue, and Cell ...
Cytotoxicity assay is the essential first step for the development of ADCs. It provides a tool to select promising ADC candidates, predict in vivo efficacy, and ...