NotI | NEB
WebProduct Information. NotI has a High Fidelity version NotI-HF ® ( NEB #R3189 ). High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity.
NotI (10 U/μL) - Thermo Fisher Scientific
WebThermo Scientific™. NotI (10 U/μL) Catalog number: ER0591. Related applications: Restriction Enzyme Cloning. Technical Support Customer Service. Recurring order eligible. Learn more » Save to list. Add to cart. Request bulk or custom quote. Product Overview. Figures. Recommendations. Documents. FAQ.
NotI-HF® | NEB
WebProduct Information. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity.
Structures of the rare-cutting restriction endonuclease NotI reveal …
WebThe structure of the rare-cutting restriction endonuclease NotI, which recognizes the 8 basepair target 5′-GCGGCCGC-3′, has been solved with and without bound DNA. Because of its specificity (recognizing a site that occurs once per 65kb), NotI is used to generate large genomic fragments and to map DNA methylation status.
NotI Is Not Boring - ScienceDirect
WebApr 8, 2008 · NotI is a dimer, as commonly observed for orthodox restriction enzymes, which interacts with palindromic recognition sites. Each subunit shows a typical restriction endonuclease-like fold, consisting of a central five-stranded β sheet flanked by two α …
FastDigest NotI - Thermo Fisher Scientific
WebFeatures. • 100% activity of all FastDigest enzymes in the universal buffer. • 100% buffer compatibility with downstream applications. • Complete digestion in 5–15 minutes. • Direct loading on gels. • No star activity. • 176 FastDigest enzymes available. Applications. • Molecular cloning. • Restriction site mapping. • Genotyping.
NotI Is Not Boring: Structure - Cell Press
WebApr 8, 2008 · NotI Is Not Boring. Crystal structures of the restriction endonuclease NotI in free and DNA bound forms, presented in this issue of Structure (. Lambert et al., 2008. ), provide a unique insight into the structural details of 8 base pair sequence recognition by the restriction enzyme.
Engineering a rare-cutting restriction enzyme: genetic screening …
WebFeb 2, 2006 · Abstract. Restriction endonucleases (REases) with 8-base specificity are rare specimens in nature. NotI from Nocardia otitidis-caviarum (recognition sequence 5′-GCGGCCGC-3′) has been cloned, thus allowing for mutagenesis and screening for enzymes with altered 8-base recognition and cleavage activity.
The apparent specificity of NotI (5'-GCGGCCGC-3') is enhanced ... - PubMed
WebMar 30, 1990 · PMID: 2111266. DOI: 10.1016/0378-1119 (90)90065-y. Abstract. The restriction endonuclease (ENase) NotI is blocked by methylation within its recognition sequence at 5'-GCGGCmCGC-3'. This sensitivity to methylation can be used to enhance the specificity of NotI in vivo and in vitro.
Compatible Cohesive Ends and Generation of New Restriction Sites
WebGenerate new restriction sites by ligation of DNA fragments with compatible cohesive or blunt ends.
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